Cells thus stained are filled by microcrystallization of silver chromate.
2.
Only when no chloride ( or any halogen ) is left will silver chromate ( red-brown ) form and precipitate out.
3.
The black reaction consisted in fixing silver chromate particles to the neurilemma ( the neuron membrane ) by reacting silver nitrate with potassium dichromate.
4.
This is for neutral pH . In very acidic pH, the silver chromate is soluble, and in alkaline pH the silver precipitated as hydroxide.
5.
Notwithstanding his work in psychiatry, Simarro is largely remembered for a contribution made in histology when he developed a silver bromide modification of Camillo Golgi's silver chromate technique.
6.
The classic Golgi stain uses potassium dichromate and silver nitrate to fill selectively with a silver chromate precipitate a few neural cells ( neurons or glia, but in principle any cells can react similarly ).
7.
This so-called silver chromate impregnation procedure stains entirely or partially the cell bodies and neurites of some neurons-dendrites, axon-in brown and black, allowing researchers to trace their paths up to their thinnest terminal branches in a slice of nervous tissue, thanks to the transparency consequent to the lack of staining in the majority of surrounding cells.
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